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Genetic Engineering
Curriculum
The Course Curriculam
Week 1: Introduction to Genetic Engineering
Basics of genetics: DNA, RNA, and protein synthesis.
History and scope of genetic engineering.
Applications in medicine, agriculture, and industry.
Hands-on Practice: Extract DNA from plant cells in a lab simulation.
Week 2: Molecular Biology Techniques
Gene cloning: Restriction enzymes and ligases
Polymerase Chain Reaction (PCR): Principles and applications.
Gel electrophoresis: DNA analysis and visualization.
Hands-on Practice: Perform virtual PCR and gel electrophoresis using online tools.
Week 3: Genetic Modification in Organisms
Transgenic organisms: Principles and creation.
CRISPR-Cas9 technology: Mechanism and applications.
Ethical considerations in genetic engineering.
Hands-on Practice: Design a CRISPR experiment for a simple genetic modification.
Week 4: Gene Expression and Regulation
Basics of gene expression: Transcription and translationvvvvv
Methods to study gene expression: Microarrays and RT-PCR
Control of gene expression: Operons and epigenetics.
Hands-on Practice: Analyze a gene expression dataset using bioinformatics tools.
Week 5: Genetic Engineering in Medicine
Gene therapy: Techniques and applications.
Genetically engineered vaccines and pharmaceuticals.
Personalized medicine and genetic testing.
Hands-on Practice: Simulate a gene therapy experiment using case studies.
Week 6: Genetic Engineering in Agriculture and Industry
Development of genetically modified crops: Traits and benefits.
Industrial applications: Enzyme production and biofuels.
Environmental impacts and biosafety concerns
Hands-on Practice: Design a genetic modification for increased crop yield.
Week 7: Computational Tools in Genetic Engineering
Introduction to bioinformatics and genetic databases (e.g., GenBank)
DNA sequence alignment and analysis.
Protein structure prediction and analysis
Hands-on Practice: Use BLAST to analyze a DNA sequence.
Week 8: Final Project and Emerging Trends
Final Project: Propose a genetic engineering solution for a real-world problem (e.g., a genetic disorder, crop resistance).
Emerging trends: Synthetic biology, gene editing in humans, and cloning.
Ethical, legal, and social implications of genetic engineering.
Presentation and feedback session.
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